ii. Electro Fusion
Recently, mild electrical stimulation is being used to fuse protoplasts. This technique is known as electrofusion of protoplasts. Two glass capillary microelectrode are placed in contact with the protoplasts. An electric field of low strength (10kv m-3) give rise to dielectrophoretic pole generation within the protoplast suspension. This lead to pearl chain arrangement of protoplasts. The number of protoplasts within a pearl chain depends upon the population density of the protoplast and the distance between the electrodes. Subsequent application of high intensity electric impulse ( 100kv m-3) for some microseconds results in the electric breakdown of membrane and subsequent fusion.
